Biostatistics and Microbiology
This “nuts and bolts” book provides a condensation of biostatistical methods that applied microbiology researchers need to perform data analyses. Based on the author’s more than two decades of applied research and teaching experience, it is presented in a straight-forward manner, applicable by practicing microbiologists with minimal backgrounds in mathematics. All methods rely only on the use of a basic hand-held calculator. The overriding goal of this book is to ground one’s microbiological expertise and experience in one’s research pursuits, using biostatistics not as a black box, but as a tool. The book begins with a broad discussion of the experimental process, leading to one-sample, two-sample, and multi-sample comparisons. Each regression analysis will be presented, as well as an example. Nonparametric methods will be discussed, as well as when they should be applied. Finally, special topics of statistics in microbiology (i.e., viral, plant, animal, human, food, industrial and microbial microbiology) will be presented.
The growing role of chemistry, combined with the contribution of chemical and pharmaceutical industries to the science of mankind, are continuously advancing. The system for training researchers in the various areas of chemistry has maintained a largely traditional and single disciplinary focus, although many investigators have broadened their selection of research techniques to encompass organized and integrated programs. As a multidisciplinary, Chemical Biology is one of the fastest expanding areas of science during this century. The interdisciplinary study of molecules in living systems at a health science campus aids the integration of the traditional disciplines of chemistry and biology. This is achieved through understanding the molecular mechanisms of biological processes, which provides the opportunity to manipulate them in a defined and predictable way. Looking forward to the next century, we will witness many more remarkable achievements within Chemistry with its application to different fields, resulting in countless benefits to human health and standards of living. This book will be an excellent addition to the fields of Chemistry and Biology, in terms of both academic depth and scientific range. The focus of this book is on the chemical and molecular aspects of Chemical Biology. The interpretation of Chemistry in Biology is to some extent broader than what is commonly referred to as Chemical Biology, as it also includes topics associated with molecular biology, biochemistry, ecology, semiochemicals, and environmental and material sciences. Chemical biologists may be especially interested in chapters concerning analytical chemistry, biochemical technology, bioinformatics, catalysis and nanotechnology, carbohydrate chemistry, bioorganic and bioinorganic chemistry. The Eurasia Conference is a key component in realising the development and advancement of chemical sciences in Asia, in cooperation with European countries, as originally initiated by Prof. Dr. Hitoshi Ohtaki. In belonging to the 9th Eurasia Conference, this book encapsulates the progress being made in the field of Chemical Biology, and is therefore a fitting tribute to Prof. H. Ohtaki’s memory.
A protein requires its own three-dimensional structure for its biological activity. If a chemical agent is added, the biological activity is lost, and the three-dimensional structure is destroyed to become a random coil state. But when the chemical agent is removed, the biological activity is recovered, implying that the random coil state turns back into the original complex structure spontaneously. This is an astonishing event. "The Physical Foundation of Protein Architecture" is intended to solve this mystery from the physicochemical basis by elucidating the mechanism of various processes in protein folding. The main features of protein folding are shown to be described by the island model with long range hydrophobic interaction which is capable of finding the specific residue, and the lampshade criterion for disulfide bonding. Various proteins with known structure are refolded, with the purpose of uncovering the mechanism of protein folding. In addition, ab initio method for predicting protein structure from its amino acid sequence is proposed.Click here to download the book
Major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors. In "HIV Protocols, Second Edition", expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, "HIV Protocols, Second Edition" is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1.Click here to download the book